摘要
目的 高频超声评价小鼠不同周龄时心脏结构及功能的改变。方法 对10只雄性C57BL/6小鼠于12、21、38周龄时行超声心动图检查，观察常规超声指标室间隔舒张末期厚度（IVS;d）、左室后壁舒张末期厚度（LVPW;d）、左室舒张末期内径（LVID;d）、左室收缩末期内径（LVID;s）、射血分数（EF）、缩短率（FS）、二尖瓣射血时间（DT）、等容舒张期（IVRT）；斑点追踪成像测量左室长轴整体及节段纵向应变的变化。结果 与12周龄比较，38周龄时小鼠IVS;d显著增大（p<0.05），而左室整体纵向应变、室间隔纵向应变则明显降低（p <0.05）。结论 应用高频超声及斑点追踪技术可以较早地获得不同周龄正常小鼠心脏结构及功能的变化，为探讨小鼠心脏模型的研究及心脏疾病的防治提供参考依据。
关键词： 高频超声心动图；斑点追踪成像技术；心脏结构和功能；增龄性改变
Abstract
Objective To evaluate the changes in cardiac structure and function of mice at different ages using high-frequency ultrasound. Methods Ten C57BL/6 mice were subjected to echocardiography at 12, 21, and 38 weeks of age to observe conventional ultrasound indicators such as interventricular septal end diastolic thickness (IVS; d), left ventricular posterior wall end diastolic thickness (LVPW; d), left ventricular end diastolic diameter (LVID; d), left ventricular end systolic diameter (LVID; s), ejection fraction (EF), shortening rate (FS), mitral valve ejection time (DT), and isovoluminous diastole (IVRT); Spot tracking imaging was used to measure the overall and segmental longitudinal strain changes in the long axis of the left ventricle. Results Compared with 12 weeks of age, IVS; d was significantly increased (p<0.05), while the overall longitudinal strain of the left ventricle and the longitudinal strain of the interventricular septum significantly decreased in mice at 38 weeks of age (p<0.05). Conclusion s The application of high-frequency ultrasound and spot tracking technology can identify early changes in the structure and function of normal mouse hearts at different weeks of age, providing reference basis for exploring mouse heart models and the prevention and treatment of heart diseases.
Key words： High frequency echocardiography; Spot tracking imaging technology; Heart structure and function; Age-related changes
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